Adaptive optics enables 3D STED microscopy in aberrating specimens.
نویسندگان
چکیده
Stimulated emission depletion (STED) microscopy allows fluorescence far-field imaging with diffraction-unlimited resolution. Unfortunately, extending this technique to three-dimensional (3D) imaging of thick specimens has been inhibited by sample-induced aberrations. Here we present the first implementation of adaptive optics in STED microscopy to allow 3D super-resolution imaging in strongly aberrated imaging conditions, such as those introduced by thick biological tissue.
منابع مشابه
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ورودعنوان ژورنال:
- Optics express
دوره 20 19 شماره
صفحات -
تاریخ انتشار 2012